Peptide Repertoire-Based Anchor Motif

An application to define HLA binding motifs

How to use PRBAM v1.0

  1. The user must select the MHC-I or HLA-DR module.
  2. Select a peptide list (text or fasta file). The peptide sequences must be in one-letter amino acid code. Only the 20 regular amino acids are considered for the final score.
  3. Click on the “Submit” button.

Allowed file formats

Plain text file (example.txt)

            [...]
            QVAEYKQLKDTLNRIPS
            VAEYKQLKDTLNRIPS
            EEFLSFLHPEHSRG
            KGRLDYLSSLKVKGL
            IRPDGEKKAYVRLAPDYDALDVA
            EKKAYVRLAPDYDALD
            EKKAYVRLAPDYDAL
            HHTFYNELRVAPEEHPV
            [...]

Fasta file (example.fasta)


            [...]
            >sequence 12
            QVAEYKQLKDTLNRIPS
            >sequence 13
            VAEYKQLKDTLNRIPS
            >sequence 14
            EEFLSFLHPEHSRG
            >sequence 15
            KGRLDYLSSLKVKGL
            >sequence 16
            IRPDGEKKAYVRLAPDYDALDVA
            >sequence 17
            EKKAYVRLAPDYDALD
            >sequence 18
            EKKAYVRLAPDYDAL
            >sequence 19
            HHTFYNELRVAPEEHPV
            [...]

Example

Select the module (MHC-I or HLA-DR) and select a peptide list (text or fasta file). Then click on Submit button.



Chart tab shows a representation of the DMP values of each amino acid in each position.



Table tab shows the DMP values of each amino acid in each position in a table format.



Cores tab shows the list of all peptides processed by PRBAM. The selected cores are highlighted and listed with their corresponding scores.



All data can be downloaded by clicking on Download button.